MYAT THU THU WIN
Asian Institute of Medicine Science and Technology (AIMST)
Title: Importance of Dosage effect in Clinical Trials
Biography
Biography: MYAT THU THU WIN
Abstract
Statement of the Problem: Therapeutic effect can be obtained by standard dosage and toxicity can occur because of over dosage. It should be taken awareness in doing clinical trials and research to get effective drug response for a particular disease or outcome. It is difficult to know the estimation of dosage in human directly so we do from preclinical research to clinical trial. In this study, I monitored different dosages of drug Metformin, well known antidiabetic agent, on lifespan of Caenorrhabditis elegans (C. elegans) (From worm to human).
Methodology: Lifesapn of C. elegans N2 Bristol wild type was monitored by using liquid culture medium in 96 well plates. 100 worms for each group were cultured in S complete media containing ampicillin (50 μg/ml) and amphotericin B (0.1 μg/ml). We used 50 μg/ml 5-fluoro-2’- deoxyuridine (5-FUdR) in well to prevent hatching. All experiments were performed at 20ºC for 2 times. C. elegans were fed alive E. coli (108 cfu/ml) and added Metformin 1 mM, 5 mM and 10 mM at day 3. After the wells were shaken for 3 minutes, worm survival was checked every other day under an inverted microscope at 50x or 100x magnification.
Findings: Different dosages of Metformin1 mM, 5 mM increase the worm survival in compared to wild type worm in this study (data not shown). Increasing the dose of Metformin more than 10mM may not increase the longevity and reduce lifespan of worms when compared to the group of worms exposed to 50 mM Metformin.
Conclusion & Significance: The longevity effect of Metformin in worms may be useful as antiaging agent in human but needs more clinical trials to explore the effect of Metformin. It was concluded that the higher dosage would not produce more increase in longevity and reduce the lifespan of worms which will be considered as important factor in clinical trials.